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. 1984 Dec 11;12(23):8951-70.
doi: 10.1093/nar/12.23.8951.

Homologous versus heterologous gene expression in the yeast, Saccharomyces cerevisiae

C Y Chen, H Oppermann, R A Hitzeman
Free PMC article

Homologous versus heterologous gene expression in the yeast, Saccharomyces cerevisiae

C Y Chen et al. Nucleic Acids Res. .
Free PMC article

Abstract

DNA sequences normally flanking the highly expressed yeast 3-phosphoglycerate kinase (PGK) gene have been placed adjacent to heterologous mammalian genes on high copy number plasmid vectors and used for expression experiments in yeast. For many genes thus far expressed with this system, expression has been 15-50 times lower than the expression of the natural homologous PGK gene on the same plasmid. We have extensively investigated this dramatic difference and have found that in most cases it is directly proportional to the steady-state levels of mRNAs. We demonstrate this phenomenon and suggest possible causes for this effect on mRNA levels.

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