The Nicotiana chloroplast genome. IX. Identification of regions active as prokaryotic promoters in Escherichia coli

Abstract

The galK-expression plasmid vector system pKO1 has been used to clone Nicotiana chloroplast (ct) promoters that function in Escherichia coli. The randomly cloned promoter-containing restriction fragments have been located on the ct genome and originate both from those regions encoding ribosomal and transfer RNAs and from locations elsewhere on the ct genome. The results provide the first demonstration that sequences which function as prokaryotic promoters exist in the ct genome.