Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Dec;4(12):2837-42.
doi: 10.1128/mcb.4.12.2837-2842.1984.

Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae

K A Krzywicki, M C Brandriss

Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae

K A Krzywicki et al. Mol Cell Biol. 1984 Dec.

Abstract

The PUT2 gene, believed to encode delta 1-pyrroline-5-carboxylate dehydrogenase, has been completely sequenced. The DNA contains an open reading frame of 1,725 base pairs encoding a protein of 575 amino acids. Transcript mapping with both S1 nuclease and primer extension methods revealed numerous initiation sites of RNA synthesis 50 to 80 base pairs downstream from several TATA boxes. The deduced amino acid sequence of delta 1-pyrroline-5-carboxylate dehydrogenase contains a highly basic amino terminus that may serve as the signal sequence that targets this protein to the mitochondrion.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
    1. Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 - PubMed
    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1496-500 - PubMed
    1. Nature. 1981 Jan 22;289(5795):244-50 - PubMed

Publication types

MeSH terms

Substances

Associated data