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. 1984 Dec;4(12):2929-31.
doi: 10.1128/mcb.4.12.2929-2931.1984.

Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells

K Blochlinger, H Diggelmann

Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells

K Blochlinger et al. Mol Cell Biol. 1984 Dec.

Abstract

The DNA coding sequence for the hygromycin B phosphotransferase gene was placed under the control of the regulatory sequences of a cloned long terminal repeat of Moloney sarcoma virus. This construction allowed direct selection for hygromycin B resistance after transfection of eucaryotic cell lines not naturally resistant to this antibiotic, thus providing another dominant marker for DNA transfer in eucaryotic cells.

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References

    1. Gene. 1983 Nov;25(2-3):179-88 - PubMed
    1. Nature. 1984 May 3-9;309(5963):82-5 - PubMed
    1. EMBO J. 1982;1(11):1313-7 - PubMed
    1. Nucleic Acids Res. 1984 May 11;12(9):3873-93 - PubMed
    1. Virology. 1973 Apr;52(2):456-67 - PubMed

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