Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Sep;143(3):1519-23.
doi: 10.1128/jb.143.3.1519-1523.1980.

Selection of Escherichia coli K-12 chromosomal mutants that prevent expression of F-plasmid functions

P Silverman, K Nat, J McEwen, R Birchman

Selection of Escherichia coli K-12 chromosomal mutants that prevent expression of F-plasmid functions

P Silverman et al. J Bacteriol. 1980 Sep.

Abstract

Chromosomal mutants of Escherichia coli deficient in the expression of F-plasmid functions were selected by mutagenizing F- cells, introducing an F' plasmid into the mutagenized cells by conjugation, and identifying transconjugants resistant to the donor-specific bacteriophage Q beta by a simple spray test. All but 1 of 25 mutants were defective in an extracellular stage of Q beta infection, suggesting that they fail to elaborate F-pili. At least six of these were also deficient as deoxyribonucleic acid donors. More than half of the mutants appear to be altered in peviously undetected chromosomal genes required for the expression of F-related cellular functions.

PubMed Disclaimer

References

    1. Biochem Biophys Res Commun. 1967 May 5;27(3):412-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 May;60(1):160-7 - PubMed
    1. J Bacteriol. 1971 May;106(2):529-38 - PubMed
    1. Bacteriol Rev. 1972 Dec;36(4):525-57 - PubMed
    1. Bacteriol Rev. 1972 Dec;36(4):587-607 - PubMed

Publication types