doi: 10.1073/pnas.77.11.6521.
Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322
- PMID: 6109282
- PMCID: PMC350317
- DOI: 10.1073/pnas.77.11.6521
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Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322
Proc Natl Acad Sci U S A.
1980 Nov.
Abstract
The Escherichia coli replication factor Y has been characterized as a phi X174 (+) strand specific DNA-dependent phosphohydrolase. In conjunction with other E. coli replication proteins, factor Y is involved in the formation of heterogeneous primers that are elongated by the E. coli DNA polymerase III elongation machinery. We report here that the heat-denatured DNAs of plasmids pBR322 and ColE1 serve as effectors for the hydrolysis of ATP by factor Y. The DNA sequences of pBR322 responsible for factor Y effector activity have been localized. Two separate regions of the pBR322 chromosome support Y ATPase activity. These sequences are near the replication origin and are located on opposite DNA strands.
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