Thermal inactivation of acetyl-CoA synthetase in the electric organ of Torpedo marmorata

Abstract

Using thermal inactivation, further characterization studies on acetyl-CoA synthetase found in isolated nerve endings and in mitochondria obtained from the electric organ of Torpedo marmorata were made. They confirm different properties of the two enzymatic forms. Moreover, the acetyl-CoA synthetase activity found in the cytosol of disrupted synaptosomes exhibits a homogeneous thermic inactivation curve which corresponds to a single enzymatic form. It has the same localization as choline acetyltransferase.