Regulatory isozymes of 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase in the cyanobacterium Anabaena sp. strain ATCC 29151

Abstract

Two regulatory isozymes of 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase from the cyanobacterium Anabaena sp. strain ATCC 29151 were separated via gel filtration. One isozyme (Mr = 205,000) was sensitive to phenylalanine inhibition, and the other (Mr = 60,000) was sensitive to tyrosine inhibition. The tyrosine-sensitive isozyme from wild-type cells was unstable to gel filtration, whereas the corresponding isozyme from an analog-resistant mutant was insensitive to tyrosine inhibition and was stable.