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. 1983 Mar 12;1(8324):555-8.
doi: 10.1016/s0140-6736(83)92811-8.

A dot hybridisation assay for detection of rotavirus

J Flores, E Boeggeman, R H Purcell, M Sereno, I Perez, L White, R G Wyatt, R M Chanock, A Z Kapikian

A dot hybridisation assay for detection of rotavirus

J Flores et al. Lancet. .

Abstract

A "dot" hybridisation technique for the detection of rotavirus in stools and other biological materials is described. The assay is based on the in-situ hybridisation of labelled single-stranded RNA probes, obtained by in-vitro transcription of rotavirus particles, to heat-denatured rotavirus RNA immobilised on nitrocellulose membranes. The method is highly specific and allows for the detection of as little as 8 pg of viral RNA. Its use for the detection of rotavirus in stool suspensions and rectal swabs obtained from children with diarrhoea may facilitate epidemiological studies of rotavirus gastroenteritis.

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