Glutamate activated postsynaptic channels in crayfish muscle investigated by noise analysis

Abstract

Excitatory synaptic channels in crayfish muscle were investigated under various experimental conditions. Small muscle fibres of length l less than or equal to 0.6 mm were voltage clamped, spatial control of the voltage being sufficient up to at least 500 Hz. Excitatory synaptic current was induced by superfusion of glutamate. The power density spectra of this current could be fitted by single component Lorentz curves. The analysis revealed a mean open time tau noise = 0.93 ms and a conductance gamma = 32.3 pS of the glutamate operated ion channels (membrane potential E = -60 mV, temperature T = 8 degrees C). Both the conductance gamma and the channel closing rate alpha = tau -1 noise increased significantly with temperature (Q10 approximately 2). The temperature dependence of gamma and alpha could be described by Arrhenius equations with the temperature independent activation energies E gamma = 42.3 kJ/mol and E alpha = 50.2 kJ/mol. alpha also dependent on the membrane potential, increasing about e-fold when the membrane was hyperpolarized by 120 mV. The potential dependence varied considerably from fibre to fibre. The mean channel open time tau noise agreed with the time constant of decay tau (sEPSC) of spontaneous excitatory postsynaptic currents (sEPSCs).