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. 1983 Dec;156(3):1052-8.
doi: 10.1128/jb.156.3.1052-1058.1983.

Genetic mapping and transcriptional orientation of the fimD gene

C S Freitag, B I Eisenstein

Genetic mapping and transcriptional orientation of the fimD gene

C S Freitag et al. J Bacteriol. 1983 Dec.

Abstract

Expression of type 1 fimbriae in Escherichia coli exhibits phase variation whereby individual cells can alternate between states of organelle expression and nonexpression. Strains carrying fim-lac operon fusions in which lac operon expression is under the control of a fim promoter undergo Lac+ in equilibrium Lac- phase variation. We have determined the genetic map location and direction of transcription of a fim-lac operon fusion which was obtained by insertion of lac into a locus we have named fimD. We found the gene order to be as follows: valS fimD uxuA serB. The direction of transcription of fimD was found to be clockwise on the E. coli chromosome.

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References

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