doi: 10.1016/0167-4838(83)90239-x.
The competition between adenosine triphosphate and inorganic pyrophosphate for myosin and its suppression by substoichiometric actin concentrations
- PMID: 6140953
- DOI: 10.1016/0167-4838(83)90239-x
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The competition between adenosine triphosphate and inorganic pyrophosphate for myosin and its suppression by substoichiometric actin concentrations
Biochim Biophys Acta.
.
Abstract
Inorganic pyrophosphate (PPi) inhibits not only Mg2+-ATPase activity of myosin subfragment 1 (S-1) but abolishes also the ATP-induced increment of tryptophan fluorescence of subfragment 1. At the concentrations used (25-50 micron ATP, 12 mm PPi) these effects of PPi were abolished by substoichiometric actin concentrations (approx. 0.1 microM actin vs. approx. 1 microM S-1), where ATPase activity was barely stimulated by actin.
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