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. 1984 May;158(2):530-4.
doi: 10.1128/jb.158.2.530-534.1984.

Identification of a glutaminyl-tRNA synthetase mutation Saccharomyces cerevisiae

A P Mitchell, S W Ludmerer

Identification of a glutaminyl-tRNA synthetase mutation Saccharomyces cerevisiae

A P Mitchell et al. J Bacteriol. 1984 May.

Abstract

Saccharomyces cerevisiae glutaminyl-tRNA synthetase mutants were isolated through systematic screening of tight Gln- derivatives of a leaky glutamine auxotroph. These mutations define a single nuclear gene, GLN4. The gln4-1 mutation is specific for Gln-tRNA synthetase and shows a dosage effect in heterozygous diploids. The wild-type Gln-tRNA synthetase exhibits a Km for glutamine of 25 microM; the gln4-1 mutation increases this value 20-fold. These observations strongly suggest that GLN4 encodes the Gln-tRNA synthetase.

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