In vitro translation of Plasmodium falciparum proteins

Abstract

RNA was prepared from cultured Plasmodium falciparum using ribonucleoside-vanadyl ribonuclease inhibitors and assayed in a rabbit reticulocyte, cell-free lysate translation system. Discrete proteins, ranging from less than 10,000 to greater than 200,000 daltons, were synthesized. Some of these proteins were precipitated by immune serum and were similar to those metabolically synthesized. Purified poly [A] mRNA was also translated and differences between the fractions which either bind or pass through a poly [U] Sepharose column are described.