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. 1984 Oct;160(1):438-41.
doi: 10.1128/jb.160.1.438-441.1984.

Single, chemically defined sporulation medium for Bacillus subtilis: growth, sporulation, and extracellular protease production

J H Hageman, G W Shankweiler, P R Wall, K Franich, G W McCowan, S M Cauble, J Grajeda, C Quinones

Single, chemically defined sporulation medium for Bacillus subtilis: growth, sporulation, and extracellular protease production

J H Hageman et al. J Bacteriol. 1984 Oct.

Abstract

The composition and application of a single, chemically defined medium or growth and sporulation of Bacillus subtilis is described. At 37 degrees C cells grew with a doubling time of about 40 min; cultures attained near-maximal spore formation (70 to 80% by 12 h after the end of exponential growth and produced 1 X 10(9) to 2 X 10(9) heat-resistant free spores at 24 h. Dipicolinic acid production was completed between 7 and 11 h. Cells grown in the single, chemically defined medium excreted levels of serine and neutral proteases comparable to those excreted in nutrient broth medium.

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