Characterization of ethoxyresorufin O-de-ethylase in grey seal Halichoerus grypus

Abstract

Ethoxyresorufin O-de-ethylase (EROD), a typical mixed function oxidase enzyme, was studied in liver and kidney of adult and pup grey seals, Halichoerus grypus. EROD activity was present in most liver samples but was only barely detectable in a few of the kidney samples. Liver EROD activity was greatest at pH 7.5-8.0; activity increased with temperature from 20 degrees C to a plateau starting at about 33 degrees C and extending to 37 degrees C, and fell off sharply above 37 degrees C. Km values ranged from 3 X 10(-7) to 3 X 10(-6)M; Vmax ranged from 5 X 10(-12) to 1 X 10(-10) moles product formed X min-1 X mg protein-1. Liver EROD activity was not detectable in pups less than 1 day old, and activity increased with age in pups up to 5-12 days old. Adults had greater EROD activity than did pups.