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. 1984 Oct;20(10):780-95.
doi: 10.1007/BF02618294.

Rat liver epithelial cell cultures in a serum-free medium: primary cultures and derived cell lines expressing differentiated functions

M Chessebeuf, P Padieu

Rat liver epithelial cell cultures in a serum-free medium: primary cultures and derived cell lines expressing differentiated functions

M Chessebeuf et al. In Vitro. 1984 Oct.

Abstract

Rat liver epithelial cells explanted in a serum-free medium (SFM) composed of Ham's F10 basal medium plus free fatty acids adsorbed on bovine albumin gave successful rise to primary cultures and then to long-term cell lines that expressed liver functions; induction of L-tyrosine aminotransferase by glucocorticoids, hepatic pattern of progesterone metabolism, and biosynthesis of murine primary bile acids; chenodeoxycholic and cholic acid common to higher vertebrates and alpha-muricholic acid specific of the rat bile.

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