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. 1980;61(2):248-50.
doi: 10.1159/000232441.

Production of monoclonal mouse IgE antibodies with DNP specificity by hybrid cell lines

I Böttcher, M Ulrich, N Hirayama, Z Ovary

Production of monoclonal mouse IgE antibodies with DNP specificity by hybrid cell lines

I Böttcher et al. Int Arch Allergy Appl Immunol. 1980.

Abstract

IgE anti-DNP antibody-secreting hybridomas were obtained by polyethylene glycol fusion of murine myeloma cells with spleen cells from mice, primed with DNP-KLH and challenged with DNP-Nippostrongylus brasiliensis extract. The in vivo and in vitro continuously growing hybridomas are producing high amounts of monoclonal IgE anti-DNP antibodies, which are heat-labile, hapten-specific and have rat mast cell sensitizing capacity.

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