doi: 10.1073/pnas.77.1.563.
Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines
- PMID: 6153797
- PMCID: PMC348313
- DOI: 10.1073/pnas.77.1.563
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Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines
Proc Natl Acad Sci U S A.
1980 Jan.
Abstract
Spleen cells from mice immunized with purified carcinoembryonic antigen (CEA), an important tumor marker of human carcinomas, were fused with the mouse myeloma cell line P3-NSI/1-Ag4. Out of the 400 hybrids obtained, 2 secreted antibodies reacting specifically with two different antigenic determinants present on CEA molecules. They were cloned and established as permanent hybridoma cell lines. These antibodies, which have relatively high-affinities and can be produced in unlimited amounts, will be useful both for the immunochemical characterization of CEA and as a standard reagent for the identification of this antigen in human tissues and body fluids.
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