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. 1980 Jan;77(1):653-7.
doi: 10.1073/pnas.77.1.653.

Method for preparing cultures of central neurons: cytochemical and immunochemical studies

J Sotelo, C J Gibbs Jr, D C Gajdusek, B H Toh, M Wurth

Method for preparing cultures of central neurons: cytochemical and immunochemical studies

J Sotelo et al. Proc Natl Acad Sci U S A. 1980 Jan.

Abstract

We report a simplified method for culturing fetal central nervous system cells predominantly inducing neurons that grow, differentiate, and live in vitro for as long as 10 weeks. These central nervous system cells form a confluent cell culture in which about 80% of the cells are fully differentiated neurons producing interconnecting axons and dendrite processes and live upon a sparse underlying population of fibrillary and protoplasmic astrocytes, oligodendrocytes, and fibroblasts. Morphological and cytochemical characteristics of these cell types, based on immunofluorescent cell specific markers and silver staining of neurons, are presented.

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References

    1. Brain Res. 1969 Jan;12(1):180-99 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2268-72 - PubMed
    1. Brain Res. 1975 Jun 6;90(1):1-21 - PubMed
    1. J Neurophysiol. 1977 Sep;40(5):1132-50 - PubMed
    1. Brain Res. 1977 Oct 21;135(1):25-36 - PubMed

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