[Some features of nuclear genome transcription at early stages of liver regeneration. Nuclear DNA-like RNAs, complementary to uniquie and repeating sequences of DNA]

Abstract

Transcription of unique and repeating sequences of DNA at early stages of liver regeneration after partial hepatectomy was studied. The total nuclear dRNA (tdRNA) and its two fractions (dRNA63O and dRNA85O) from the control and regenerating liver were subjected to hybridization with the excess of DNA. One hour after partial hepatectomy the kinetics of tdRNA hybridization did not differ from the controls; 3 hrs after operation the rate of unique transcripts hybridization was decreased. The rate of dRNA63O hybridization was shown to be considerably decreased 3 hrs after partial hepatectomy, while the kinetics of hybridization of dRNA85O remained unchanged. It was assumed that the changes of dRNA hybridization during the first few hours after partial hepatectomy are due to a significant increase in the amount of copies of some unique transcripts.