[Staining of semithin Durcupan sections]

Abstract

The author described a modification of a simple undemanding method of staining Durcupan section of tissues fixed in aldehydes and in osmium. Methylene blue with azure II was used for the staining. After rinsing in water the sections were counterstained with basic fuchsin, or with saphranin O. Basophil structures are stained blue or even violet, fat and erythrocytes blue-green. After counterstaining with saphranin, mucus, collagen, and glycogen come out pink, or red case fuschin is used. Elastic tissue come out violet. The colours are bright, full of contrast, and practically fast.