Comparison of synthesis and secretion of plasma albumin, fibrinogen and alpha 2-macroglobulin by slices of Morris hepatomas and rat liver

Abstract

Slices of rat liver or Morris Hepatomas 5123 GVI and 7777 were incubated for 2 h with 14C-leucine. Newly synthesized labelled proteins were determined independently in the medium and tissue homogenate, total protein after precipitation with trichloro-acetic acid, and albumin, fibrinogen and alpha 2-macroglobulin after immuno-precipitation with monospecific antisera. In comparison with control rat liver, synthesis and secretion of albumin were reduced four- to five-fold in the 2 heptomas examined. On the other hand, synthesis and secretio of fibrinogen and alpha 2-macroglobulin in Hepatomas 5123 GVI annd 7777 were similar to rat liver, especially to the liver isolated from rats with turpentine-induced local inflammation.