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. 1980 Oct 2;287(5781):411-6.
doi: 10.1038/287411a0.

Human leukocyte interferon produced by E. coli is biologically active

D V Goeddel, E Yelverton, A Ullrich, H L Heyneker, G Miozzari, W Holmes, P H Seeburg, T Dull, L May, N Stebbing, R Crea, S Maeda, R McCandliss, A Sloma, J M Tabor, M Gross, P C Familletti, S Pestka

Human leukocyte interferon produced by E. coli is biologically active

D V Goeddel et al. Nature. .

Abstract

A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection.

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