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. 1980 Sep 25;8(18):4057-74.
doi: 10.1093/nar/8.18.4057.

Synthesis of human fibroblast interferon by E. coli

D V Goeddel, H M Shepard, E Yelverton, D Leung, R Crea, A Sloma, S Pestka
Free PMC article

Synthesis of human fibroblast interferon by E. coli

D V Goeddel et al. Nucleic Acids Res. .
Free PMC article

Abstract

A cDNA library was constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences was identified by hybridization to a cDNA probe synthesized using deoxyoligonucleotide primers which hybridize to fibroblast interferon mRNA specifically. Expression plasmids were constructed which permitted the synthesis in E. coli of 8 x 10(7) units of human fibroblast interferon per liter of culture. The bacterially produced fibroblast interferon is indistinguishable from authentic human fibroblast interferon by several criteria.

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References

    1. Proc Natl Acad Sci U S A. 1970 Jan;65(1):168-75 - PubMed
    1. Nature. 1971 Sep 17;233(5316):177-82 - PubMed
    1. J Bacteriol. 1972 May;110(2):667-76 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3898-901 - PubMed

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