Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Jul;77(7):3903-7.
doi: 10.1073/pnas.77.7.3903.

Posttranscriptional regulation of an erythromycin resistance protein specified by plasmic pE194

A G Shivakumar, J Hahn, G Grandi, Y Kozlov, D Dubnau

Posttranscriptional regulation of an erythromycin resistance protein specified by plasmic pE194

A G Shivakumar et al. Proc Natl Acad Sci U S A. 1980 Jul.

Abstract

Induction of the synthesis of a plasmid-encoded polypeptide (E3) by erythromycin is known to be required for the inducible expression of resistance to the macrolide-lincosamide-streptogramin B group of antibiotics in Bacillus subtilis strains carrying the plasmid pE194. This resistance is mediated by a specific N6-dimethylation of adenine in the 23S rRNA of the large ribosomal subunit. We show in this report that E3 induction is regulated posttranscriptionally in the sense that it can occur when RNA synthesis is blocked and that induction is accompanied by an increase in the functional half-life of E3 mRNA but not of the mRNA species that code for the remaining four known pE194 polypeptides. The induction of E3 is subject to feedback regulation and involves the ribosome. Modification of the erythromycin binding site on the ribosome by methylation or by mutation interferes with induction.

PubMed Disclaimer

References

    1. J Bacteriol. 1961 May;81(5):741-6 - PubMed
    1. Antimicrob Agents Chemother. 1976 Jan;9(1):128-30 - PubMed
    1. J Bacteriol. 1971 Jun;106(3):835-47 - PubMed
    1. Antimicrob Agents Chemother. 1977 Apr;11(4):669-74 - PubMed
    1. Jpn J Microbiol. 1975 Oct;19(5):343-7 - PubMed

Publication types