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. 1980 Sep;77(9):5230-3.
doi: 10.1073/pnas.77.9.5230.

Expression of the human fibroblast interferon gene in Escherichia coli

T Taniguchi, L Guarente, T M Roberts, D Kimelman, J Douhan 3rd, M Ptashne

Expression of the human fibroblast interferon gene in Escherichia coli

T Taniguchi et al. Proc Natl Acad Sci U S A. 1980 Sep.

Abstract

We applied the method of Guarente et al. [Guarente, L., Lauer, G., Roberts, T.M. & Ptashne, M. (1980) Cell 20, 543-553] to construct plasmids that direct expression in Escherichia coli of the human fibroblast interferon (F-IF) gene. Two plasmids were recovered. One directs efficient synthesis of a protein whose primary sequence is that of pre-F-IF and the other, that of mature F-IF. Extracts of bacteria synthesizing mature F-IF display antiviral activity characteristic of human F-IF. This activity is lower than that expected from the differential rate of synthesis of the protein. We have detected no such activity in extracts of bacteria synthesizing pre-F-IF.

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