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. 1980 Sep 26;209(4464):1538-41.
doi: 10.1126/science.6159680.

Cell lineage analysis by intracellular injection of fluorescent tracers

D A Weisblat, S L Zackson, S S Blair, J D Young

Cell lineage analysis by intracellular injection of fluorescent tracers

D A Weisblat et al. Science. .

Abstract

Cell lineages during development of the leech are revealed by injection of a fluorescent peptide, rhodamine-D-peptide, into identified embryonic cells. Use of this peptide together with a nuclear stain showed a stereotypic cleavage pattern of stem cells and their progeny. Combined injection of rhodamine-D-peptide and pronase demonstrated the arrest of stem cell production in the pronase-injected teloblast.

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