doi: 10.1016/s0300-9785(80)80019-6.
Effect of fixation upon the histochemistry of glycosaminoglycans (mucopolysaccharides) in intraoral wounds
- PMID: 6160113
- DOI: 10.1016/s0300-9785(80)80019-6
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Effect of fixation upon the histochemistry of glycosaminoglycans (mucopolysaccharides) in intraoral wounds
Int J Oral Surg.
1980 Jun.
Abstract
Intraoral wounds and biochemical films were examined to determine the best method of fixation for glycosaminoglycans. Tissues fixed in 1% cetyl pyridinium chloride-10% formalin stained intensely with preparations of Alcian blue. When these tissues were treated with Streptomyces hyaluronidase and bacterial chondroitinases, staining was comparable to untreated tissues fixed in only 10% formalin. The addition of cetyl pyridinium chloride to formalin fixatives was a requirement for glycosaminoglycan histochemistry in open wounds, and presumably in other tissues that contain these complex sugars.
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