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. 1980 Jun;9(3):198-205.
doi: 10.1016/s0300-9785(80)80019-6.

Effect of fixation upon the histochemistry of glycosaminoglycans (mucopolysaccharides) in intraoral wounds

S A Alexander, M Swerdloff

Effect of fixation upon the histochemistry of glycosaminoglycans (mucopolysaccharides) in intraoral wounds

S A Alexander et al. Int J Oral Surg. 1980 Jun.

Abstract

Intraoral wounds and biochemical films were examined to determine the best method of fixation for glycosaminoglycans. Tissues fixed in 1% cetyl pyridinium chloride-10% formalin stained intensely with preparations of Alcian blue. When these tissues were treated with Streptomyces hyaluronidase and bacterial chondroitinases, staining was comparable to untreated tissues fixed in only 10% formalin. The addition of cetyl pyridinium chloride to formalin fixatives was a requirement for glycosaminoglycan histochemistry in open wounds, and presumably in other tissues that contain these complex sugars.

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