The use of pronase enhances sensitivity of the PAP method in the detection of intracytoplasmic immunoglobulins
- PMID: 6160845
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The use of pronase enhances sensitivity of the PAP method in the detection of intracytoplasmic immunoglobulins
Basic Appl Histochem.
1980.
Abstract
Application of immunocytochemistry to formalin-fixed, paraffin-embedded material tends to provide capricious results, even when employing the unlabelled antibody peroxidase-antiperoxidase (PAP) method. This study shows that treatment of tissue sections with pronase, a protease isolated from a strain of Streptomyces griseus, prior to performance of the PAP procedure greatly enhances its sensitivity. The optimum conditions for use of this enzyme in detecting intracellular immunoglobulins are given.
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