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. 1980 Jul 15;190(1):45-50.
doi: 10.1042/bj1900045.

Preparation of resealed pig lymphocyte plasma-membrane vesicles

A P Johnstone, M J Crumpton

Preparation of resealed pig lymphocyte plasma-membrane vesicles

A P Johnstone et al. Biochem J. .

Abstract

Pig lymphocyte plasma-membrane vesicles that are impermeable to large molecules were prepared by means of centrifugation on a continuous dextran gradient. They were isolated in a yield of 60--80% of the total plasma membrane recovered, and were characterized as being impermeable to macromolecules by their exclusion of inulin (mol.wt. 5200) and Dextran T10 (mol.wt. 10 000).

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References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Biochem J. 1970 Nov;120(1):133-43 - PubMed
    1. Biochim Biophys Acta. 1973 Jul 6;311(3):362-80 - PubMed
    1. J Biol Chem. 1974 Apr 10;249(7):2135-42 - PubMed
    1. Biochem Biophys Res Commun. 1974 Apr 8;57(3):787-92 - PubMed