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Comparative Study
. 1977;13(2):207-13.

Cleavage of damaged DNA by an enzymatic activity from human fibroblasts

G Zupi, R Elli, A Cianciulli, B Nicoletti, A Caputo
  • PMID: 616301
Comparative Study

Cleavage of damaged DNA by an enzymatic activity from human fibroblasts

G Zupi et al. Biochem Exp Biol. 1977.

Abstract

An endonuclease activity isolated from normal, Xeroderma Pigmentosum and De Sanctis-Cacchione fibroblasts by DNA-cellulose chromatography has been evaluated by means of sedimentation analysis both in alkaline and neutral sucrose gradients. The partially purified enzyme is active at pH 7.5 in presence of Mg++ and cleaves UV-irradiated and alkylated DNA. Under identical experimental conditions, the enzyme is not active on untreated, depurinated and heat-denaturated DNA. The specificity on pyrimidine dimers has been further investigated to understand the mechanism of action of the enzyme.

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