Sectioning and imaging hard mineral fibres in biological tissues
- PMID: 6163862
- DOI: 10.1111/j.1365-2818.1981.tb01245.x
Sectioning and imaging hard mineral fibres in biological tissues
Abstract
A technique is described which overcomes the problems associated with sectioning biological tissue containing hard mineral fibres. 0.2--0.5 micrometer thick sections were cut with a diamond knife, placed in a folding grid, conventionally stained with uranyl acetate and lead citrate and viewed at an accelerating voltage of 200 kV in the scanning transmission mode.
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