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. 1981 Apr;122(Pt 1):87-92.
doi: 10.1111/j.1365-2818.1981.tb01245.x.

Sectioning and imaging hard mineral fibres in biological tissues

N F Johnson, K Ibe

Sectioning and imaging hard mineral fibres in biological tissues

N F Johnson et al. J Microsc. 1981 Apr.

Abstract

A technique is described which overcomes the problems associated with sectioning biological tissue containing hard mineral fibres. 0.2--0.5 micrometer thick sections were cut with a diamond knife, placed in a folding grid, conventionally stained with uranyl acetate and lead citrate and viewed at an accelerating voltage of 200 kV in the scanning transmission mode.

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