Evidence for a unique human fibroblast interferon (IFN-beta 1) chromosomal gene, devoid of intervening sequences
- PMID: 6164047
- PMCID: PMC327215
- DOI: 10.1093/nar/9.3.461
Evidence for a unique human fibroblast interferon (IFN-beta 1) chromosomal gene, devoid of intervening sequences
Abstract
Direct restriction analysis of the human genome, using the Southern transfer technique and hybridization with a human fibroblast interferon (IFN-beta) complementary DNA insert probe, revealed the presence of a single gene; no additional closely related IFN-beta genes could be detected. A lambda-linked human gene library (Lawn et al., Cell, 15, 1157-1174 (1978)) was screened using the cDNA probe. Out of 600,000 recombinant phage examined, one single clone bearing interferon sequences was obtained. Restriction analysis of the relevant region revealed an identical restriction map as obtained for the IFN-beta 1 cDNA clones. No intervening sequences could be detected, either in the coding or the non-coding regions of the gene.
Similar articles
-
Nucleotide sequence of the chromosomal gene for human fibroblast (beta 1) interferon and of the flanking regions.Gene. 1981 Aug;14(3):137-43. doi: 10.1016/0378-1119(81)90109-8. Gene. 1981. PMID: 6169589
-
The structure of a thirty-six kilobase region of the human chromosome including the fibroblast interferon gene IFN-beta.Nucleic Acids Res. 1981 Jun 11;9(11):2495-507. doi: 10.1093/nar/9.11.2495. Nucleic Acids Res. 1981. PMID: 6168999 Free PMC article.
-
The absence of introns within a human fibroblast interferon gene.Nucleic Acids Res. 1981 Jan 24;9(2):247-66. doi: 10.1093/nar/9.2.247. Nucleic Acids Res. 1981. PMID: 6163136 Free PMC article.
-
Isolation and characterization of a human fibroblast interferon gene and its expression in Escherichia coli.Rev Infect Dis. 1981 Nov-Dec;3(6):1186-95. doi: 10.1093/clinids/3.6.1186. Rev Infect Dis. 1981. PMID: 6177026 Review.
-
Structure and expression of human IFN-alpha genes.Philos Trans R Soc Lond B Biol Sci. 1982 Sep 24;299(1094):7-28. doi: 10.1098/rstb.1982.0102. Philos Trans R Soc Lond B Biol Sci. 1982. PMID: 6128751 Review.
Cited by
-
Multiple human beta interferon genes.J Exp Med. 1982 Sep 1;156(3):744-55. doi: 10.1084/jem.156.3.744. J Exp Med. 1982. PMID: 6180119 Free PMC article.
-
The chromosomal location of mouse interferon alpha genes.EMBO J. 1984 Jul;3(7):1643-6. doi: 10.1002/j.1460-2075.1984.tb02023.x. EMBO J. 1984. PMID: 6745245 Free PMC article.
-
Analysis of interferon mRNA in human fibroblast cells induced to produce interferon.Proc Natl Acad Sci U S A. 1981 Dec;78(12):7426-30. doi: 10.1073/pnas.78.12.7426. Proc Natl Acad Sci U S A. 1981. PMID: 6174973 Free PMC article.
-
Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs.Nucleic Acids Res. 1982 May 25;10(10):3069-84. doi: 10.1093/nar/10.10.3069. Nucleic Acids Res. 1982. PMID: 6179042 Free PMC article.
-
Molecular pathogenesis of virus infections.Experientia. 1987 Dec 1;43(11-12):1193-6. doi: 10.1007/BF01945522. Experientia. 1987. PMID: 2826215 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
