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. 1981 Mar;78(3):1601-5.
doi: 10.1073/pnas.78.3.1601.

Partial purification and characterization of human gamma (immune) interferon

Y K Yip, R H Pang, C Urban, J Vilcek

Partial purification and characterization of human gamma (immune) interferon

Y K Yip et al. Proc Natl Acad Sci U S A. 1981 Mar.

Abstract

Human gamma (immune) interferon (IFN-gamma) was produced in lymphocyte cultures stimulated with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate) and purified phytohemagglutinin. Physicochemical analysis showed that human IFN-gamma is a glycoprotein with an isoelectric point around 8.6 and an apparent molecular weight of 58,000 +/- 3000. A purification process for IFN-gamma was developed consisting of sequential chromatographic separations on controlled-pore glass, concanavalin A-Sepharose, and Bio-Gel P-200. This purification process resulted in an increase in specific activity from about 10(4) (crude culture fluid) to an estimated 10(7) units per mg of protein with a cumulative recovery of about 40% of the IFN activity.

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