Block staining of mammalian tissues with hematoxylin and eosin
- PMID: 6166093
- DOI: 10.3109/10520298109067294
Block staining of mammalian tissues with hematoxylin and eosin
Abstract
Various mammalian tissues were stained en bloc with hematoxylin and eosin after fixation and prior to embedding in paraffin wax and sectioning. The choice of fixative is important and best results are obtained using Worcester's Fluid, a combination of saturated aqueous mercuric chloride, formaldehyde, and glacial acetic acid. After fixation, blocks of tissue up to 1.5 cm thick are stained for seven days in hematoxylin. Excess stain is removed by washing tissues in running water overnight. Tissue blocks then are dehydrated with graded concentrations of ethyl alcohols to 80% and counterstained, with further dehydration, in 0.5% spirit soluble eosin in 90% ethyl alcohol for five days. The tissue is subsequently transferred to 90% ethyl alcohol overnight to differentiate eosin staining; dehydration is completed in absolute ethyl alcohol. The blocks are cleared in in cedarwood oil and briefly in xylene prior to embedding, sectioning, and mounting. Following removal of wax by xylene, coverslips are applied. General morphological and histological features were particularly well differentiated and very selectively and reliably stained by this method.
Similar articles
-
[Modified method of staining semi-thin sections with hematoxylin and eosin].Arkh Patol. 1983;45(8):88-9. Arkh Patol. 1983. PMID: 6196005 Russian.
-
Modified hematoxylin and eosin staining method for epoxy-embedded tissue sections.Pathol Res Pract. 1989 Jun;184(6):635-8. doi: 10.1016/S0344-0338(89)80170-0. Pathol Res Pract. 1989. PMID: 2476794
-
[Hematoxylin and eosin staining of semi-thin sections of aldehyde-osmium tetroxide-fixed tissues embedded in epoxy resin "Quetol 812"].Kaibogaku Zasshi. 1984 Feb;59(1):3-8. Kaibogaku Zasshi. 1984. PMID: 6206679 Japanese. No abstract available.
-
The hematoxylin and eosin stain in anatomic pathology-An often-neglected focus of quality assurance in the laboratory.Semin Diagn Pathol. 2019 Sep;36(5):303-311. doi: 10.1053/j.semdp.2019.06.003. Epub 2019 Jun 4. Semin Diagn Pathol. 2019. PMID: 31230963 Review.
-
The wonderful colors of the hematoxylin-eosin stain in diagnostic surgical pathology.Int J Surg Pathol. 2014 Feb;22(1):12-32. doi: 10.1177/1066896913517939. Epub 2014 Jan 9. Int J Surg Pathol. 2014. PMID: 24406626 Review.
Cited by
-
The interaction of the SARS coronavirus non-structural protein 10 with the cellular oxido-reductase system causes an extensive cytopathic effect.J Clin Virol. 2005 Oct;34(2):133-9. doi: 10.1016/j.jcv.2004.12.019. J Clin Virol. 2005. PMID: 16157265 Free PMC article.
-
Longitudinal mechanical tension induces growth in the small bowel of juvenile rats.Gut. 2005 Aug;54(8):1085-90. doi: 10.1136/gut.2004.061481. Epub 2005 Apr 19. Gut. 2005. PMID: 15840689 Free PMC article.
-
An improved method for the establishment of a model of Graves' disease in BALB/c mice.Mol Med Rep. 2017 Apr;15(4):1471-1478. doi: 10.3892/mmr.2017.6181. Epub 2017 Feb 8. Mol Med Rep. 2017. PMID: 28259898 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials