[Feasibility of using human spleen cells to produce interferon]
[Article in
Russian]
- PMID: 6167069
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[Feasibility of using human spleen cells to produce interferon]
[Article in
Russian]
Vopr Virusol.
1981 Jan-Feb.
Abstract
Spleen cells of subjects who died suddenly are capable of producing large amounts of interferon in response to induction with Newcastle disease virus (NDV) and Sendai virus (1-2.5 IU per 1000 cells). The optimal conditions for interferon production by these cells include using NDV, the H strain, as an inducer in a dose of 10 ID50/cell and fresh spleen cells stored no more than 24 hours at 4-6 degrees C in a concentration of 0.5-1.0 x 10(7)/ml, with the incubation time of 20-24 hours. It is recommended that spleen cells from subjects who died suddenly be used for human interferon production.
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