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. 1981;71(4):513-20.
doi: 10.1007/BF00508377.

A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff

K Yamada, H Kitamura, Y Maseki

A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff

K Yamada et al. Histochemistry. 1981.

Abstract

A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines an alkaline phosphatase-labeled concanavalin A-5-bromo-3-indolyl phosphate, p-toluidine salt (Con A-ALP-BIPT) method with periodic acid-Schiff (PAS) sequence. With the present dual staining method, it is possible to color alpha-D-glucosyl and alpha-D-mannosyl residues blue and 1,2-glycol groups of neutral complex carbohydrates magenta. The validity of this method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.

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