Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981;71(4):513-20.
doi: 10.1007/BF00508377.

A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff

K Yamada, H Kitamura, Y Maseki

A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff

K Yamada et al. Histochemistry. 1981.

Abstract

A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines an alkaline phosphatase-labeled concanavalin A-5-bromo-3-indolyl phosphate, p-toluidine salt (Con A-ALP-BIPT) method with periodic acid-Schiff (PAS) sequence. With the present dual staining method, it is possible to color alpha-D-glucosyl and alpha-D-mannosyl residues blue and 1,2-glycol groups of neutral complex carbohydrates magenta. The validity of this method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.

PubMed Disclaimer

References

    1. Okajimas Folia Anat Jpn. 1979 Oct;56(4):193-204 - PubMed
    1. Histochemie. 1973;33(1):87-94 - PubMed
    1. Stain Technol. 1948 Jul;23(3):99-108 - PubMed
    1. Histochemie. 1968;15(1):68-78 - PubMed
    1. Histochemistry. 1977 Aug 1;53(2):143-56 - PubMed

LinkOut - more resources