Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980;1(3):157-65.
doi: 10.1016/0166-0934(80)90012-9.

Purification of reverse transcriptase from avian retroviruses using affinity chromatography on heparin-sepharose

M Golomb, A C Vora, D P Grandgenett

Purification of reverse transcriptase from avian retroviruses using affinity chromatography on heparin-sepharose

M Golomb et al. J Virol Methods. 1980.

Abstract

Reverse transcriptase from Rous sarcoma virus and avian myeloblastosis virus was purified by a rapid two-step procedure using chromatography on phosphocellulose and heparin-Sepharose. The resulting enzyme was homogeneous, had a high specific activity and was free of contaminating nucleases. This procedure has been adapted to small-scale preparation of enzyme from mutant virus containing thermolabile reverse transcriptase, and is equally suitable for large-scale enzyme purification.

PubMed Disclaimer

Publication types

LinkOut - more resources