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. 1981 Oct 29;293(5835):717-22.
doi: 10.1038/293717a0.

Expression of a human gene for interferon in yeast

R A Hitzeman, F E Hagie, H L Levine, D V Goeddel, G Ammerer, B D Hall

Expression of a human gene for interferon in yeast

R A Hitzeman et al. Nature. .

Abstract

A DNA sequence coding for mature human leukocyte interferon D (LeIF-D) was linked with DNA fragments of the 5'-flanking sequences of the Saccharomyces cerevisiae (yeast) alcohol dehydrogenase I gene in a plasmid capable of autonomous replication and selection in both yeast and Escherichia coli. Yeast cells transformed by these plasmids synthesize up to 1 x 10(6) molecules of biologically active LeIF-D per cell.

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