doi: 10.1007/BF00409453.
Changes in suspensions of human keratinocytes due to trypsin
- PMID: 6171208
- DOI: 10.1007/BF00409453
Item in Clipboard
Changes in suspensions of human keratinocytes due to trypsin
Arch Dermatol Res.
1981.
Abstract
Epidermal cell suspensions derived from keratotomed samples of normal human skin have been studied by light microscopy and by scanning and transmission electron microscope techniques. The method allows detailed inspection of the keratinocyte membranes. However, the trypsin used to obtain the suspensions limits the usefulness of the technique as some of the features observed - including invagination of desmosomes, vacuolation, and redistribution of tonofibrils - may be attributable to this proteolytic enzyme.
Similar articles
-
The experimental production of high-level intraepidermal splits.Br J Dermatol. 1980 Jan;102(1):75-83. doi: 10.1111/j.1365-2133.1980.tb05674.x. Br J Dermatol. 1980. PMID: 7378284
-
The morphology of the denuded epidermal basal cell layer of the hairless mouse after different preparation methods. A scanning and transmission electron microscopical study.Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;57(3):181-94. doi: 10.1007/BF02899080. Virchows Arch B Cell Pathol Incl Mol Pathol. 1989. PMID: 2570485
-
Apoptosis in human epidermis: a postmortem study by light and electron microscopy.Australas J Dermatol. 1986 Apr;27(1):1-5. doi: 10.1111/j.1440-0960.1986.tb00274.x. Australas J Dermatol. 1986. PMID: 2428348 No abstract available.
-
Molecular basis of the skin barrier structures revealed by electron microscopy.Exp Dermatol. 2018 Aug;27(8):841-846. doi: 10.1111/exd.13674. Epub 2018 Jun 28. Exp Dermatol. 2018. PMID: 29704884 Review.
-
Keratinization of human epidermal cells in culture.Int J Dermatol. 1979 Dec;18(10):787-96. doi: 10.1111/j.1365-4362.1979.tb04465.x. Int J Dermatol. 1979. PMID: 93587 Review. No abstract available.
Cited by
-
Pharmacotherapy of acute lung injury and the acute respiratory distress syndrome.J Intensive Care Med. 2006 May-Jun;21(3):119-43. doi: 10.1177/0885066606287045. J Intensive Care Med. 2006. PMID: 16672636 Free PMC article. Review.
-
Fractionation of Paget cells in extramammary Paget's disease.Arch Dermatol Res. 1993;285(8):502-4. doi: 10.1007/BF00376824. Arch Dermatol Res. 1993. PMID: 8274038 No abstract available.
-
Alterations in membrane fluidity during keratinocyte differentiation measured by fluorescence polarization.Cell Tissue Res. 1990 Apr;260(1):207-10. doi: 10.1007/BF00297507. Cell Tissue Res. 1990. PMID: 2340583
-
α6 Integrin (α6high)/Transferrin Receptor (CD71)low Keratinocyte Stem Cells Are More Potent for Generating Reconstructed Skin Epidermis Than Rapid Adherent Cells.Int J Mol Sci. 2017 Jan 27;18(2):282. doi: 10.3390/ijms18020282. Int J Mol Sci. 2017. PMID: 28134816 Free PMC article.