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. 1981 Nov 25;9(22):6153-66.
doi: 10.1093/nar/9.22.6153.

Antiviral activities of hybrids of two major human leukocyte interferons

P K Weck, S Apperson, N Stebbing, P W Gray, D Leung, H M Shepard, D V Goeddel

Antiviral activities of hybrids of two major human leukocyte interferons

P K Weck et al. Nucleic Acids Res. .

Abstract

Four hybrid human leukocyte interferon (LeIF or IFN-alpha) genes have been constructed by in vitro recombination of LeIF-A (IFN-alpha 2) and LeIF-D (IFN-alpha 1) genes at common restriction endonuclease sites located within their coding regions. These hybrid genes have been expressed in E. coli under trp promoter control. The interferons produced [LeIF-AD (BglII), -AD (PvuII), -DA (BglII), -DA (PvuII)] have antiviral properties distinct from the parental molecules LeIF-A and -D, varying considerably in their abilities to inhibit plaque formation by different viruses in a range of mammalian cells. All six of the cloned LeIFs exhibit the heat stability, pH 2 stability and antigenic specificity of natural leukocyte interferons.

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