Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Jul;17(7):563-9.
doi: 10.1007/BF02618453.

An autoradiographic method of detecting A. laidlawii and M. hyorhinis in cell cultures

J Keprtova, K Jurmanova, V Spurna, E Minarova, J Hofmanova, M Nebola

An autoradiographic method of detecting A. laidlawii and M. hyorhinis in cell cultures

J Keprtova et al. In Vitro. 1981 Jul.

Abstract

The autoradiographic investigation of L cells and chinese hamster cells for the presence of mycoplasmas (A. laidlawii and M. hyorhinis) using uridine/uracil (UdR/U) testing is a rapid and reliable method suitable for the serial checking of even a small number of cells. It depends on a reduced incorporation of [3H]uridine and an increased uptake of [3H]uracil into the RNA of mycoplasma-infected cells, shown in autoradiograms by the density of the grains and their distribution. Results obtained by the autoradiographic technique correspond approximately to specific activity values of RNA-infected cells after the incorporation of [3H]uridine and [3H]uracil.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(4):615-22 - PubMed
    1. Exp Cell Res. 1977 Feb;104(2):255-62 - PubMed
    1. Exp Cell Res. 1972 Sep;74(1):99-109 - PubMed
    1. Nature. 1975 Jul 24;256(5515):329-31 - PubMed
    1. In Vitro. 1976 Sep;12(9):643-8 - PubMed