Abnormal antigenic reactivity of factor VIII/von Willebrand factor subunit in variants of von Willebrand's disease

Abstract

Antibodies specific for either the subunit (MW 2.4 X 10(5)) or conformational antigenic determinants of F.VIII/vWF were prepared in order to investigate further the abnormal antigenic reactivity in variants of vWD. Goat anti-human F.VIII/vWF Fab fragments were immunoadsorbed with F.VIII/vWF subunits bound to activated thiol-Sepharose, and the "anti-subunit" antibody was eluted at pH 2.4. Antibodies that did not bind to the beads were designated as "anti-conformation" antibody. The anti-subunit specifically reacted by IRMA with the F.VIII/vWF subunits and with all subunit-containing multimers (MW 4.8 X 10(5) to greater than 15 X 10(6)). The anti-conformation antibody failed to react with F.VIII/vWF subunits but reacted with native (MW 1 to greater than 15 X 10(6)) or partially reduced (MW 4.8 X 10(5)) F.VIII/vWF. It was thus specific for antigenic sites resulting from the association of subunits in dimers or multimers of F.VIII/vWF. In eight patients with a variant of vWD (type IIA), an abnormal antigenic reactivity in plasma, characterized by a dose-response curve not parallel to that of control, was consistently observed by IRMA using the anti-subunit antibody. Six showed a normal dose-response curve with the anti-conformation antibody, and two unique patients also demonstrated an abnormal (nonparallel) response. This study (1) allows the distinction between subunit and conformational antigenic sites on F.VIII/vWF, (2) demonstrates that the decreased antigenicity in type IIA vWD is associated in all cases with an abnormality of F.VIII/vWF subunits and in some patients with an additional defect of polymerization, and (3) further emphasizes the heterogeneity of variants of vWD.