Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Feb 11;10(3):967-77.
doi: 10.1093/nar/10.3.967.

Inducer-responsive expression of the cloned human interferon beta 1 gene introduced into cultured mouse cells

S Ohno, T Taniguchi

Inducer-responsive expression of the cloned human interferon beta 1 gene introduced into cultured mouse cells

S Ohno et al. Nucleic Acids Res. .

Abstract

A hybrid plasmid was constructed from pSV2-Ecogpt (Mulligan and Berg, 1981) and the cloned 1.8 kilobase chromosomal DNA segment which contains the human interferon (HIFN-beta 1) gene and its flanking sequences. Cultured mouse FM3A cells were transfected by the hybrid plasmid and several Ecogpt+ clones were selected. Southern blot analysis of the DNA from these mouse cell clones showed that two of them contained a few copies of the 1.8 kilobase human DNA. Synthesis of mRNA characteristic of the HIFN-beta 1 mRNA increased as measured by RNA blot analysis when those clones were treated with Newcastle disease virus or with poly(I):poly(C). In addition, the induced mouse cell clones gave rise to the synthesis of human IFN-beta 1. These results suggest the presence of a unique nucleotide sequence in the 1.8 kilobase DNA which is sufficient for the inducer-responsive expression of the human IFN-beta 1 gene.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 - PubMed
    1. Cell. 1981 Feb;23(2):335-45 - PubMed
    1. Nucleic Acids Res. 1981 Mar 11;9(5):1045-52 - PubMed
    1. Gene. 1981 Aug;14(3):137-43 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 May;69(5):1203-7 - PubMed

Publication types