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. 1982;3(2):69-72.

Isolation of human alpha-1 fetoprotein (AFP) from induced abortion material

G Haller, P Linneke, P Voss
  • PMID: 6176562

Isolation of human alpha-1 fetoprotein (AFP) from induced abortion material

G Haller et al. Int J Biol Res Pregnancy. 1982.

Abstract

The isolation of alpha-1 fetoprotein (AFP) from material of an induced abortion is described. AFP is purified by anion exchange chromatography on DEAE-Sephadex A 50, affinity chromatography on Con-A-Sepharose, and preparative electrophoresis on agarose gel. The yield is about 10% of the original material. The purity was demonstrated by agarose gel electrophoresis and two-dimensional immunoelectrophoresis. The high concentration of AFP in material of induced abortion makes it particularly suitable for deriving human AFP.

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