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. 1982 Mar;103(3-6):321-6.
doi: 10.1016/0165-7992(82)90061-6.

Measurement of SCE frequencies in plants: a simple Feulgen-staining procedure for vicia faba

M J Tempelaar, M T de Both, J E Versteegh

Measurement of SCE frequencies in plants: a simple Feulgen-staining procedure for vicia faba

M J Tempelaar et al. Mutat Res. 1982 Mar.

Abstract

A relatively straightforward approach is described to obtain differential contrast in sister chromatids for SCE detection in Vicia faba after BrdUrd incorporation. The hydrolysis time of the well-known Feulgen reaction was extended to differentially degrade the DNA, the BrdUrd-substituted strands being more resistant. The procedure may easily be adapted for other plant species with large chromosomes.

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